INVIVO BROMODEOXYURIDINE INCORPORATION IN NORMAL MOUSE KIDNEY - IMMUNOHISTOCHEMICAL DETECTION AND MEASUREMENT OF LABELING INDEXES

被引:20
作者
ROBERTSON, H [1 ]
WHEELER, J [1 ]
MORLEY, AR [1 ]
机构
[1] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT MICROBIOL,NEWCASTLE TYNE NE2 4HH,ENGLAND
来源
HISTOCHEMICAL JOURNAL | 1990年 / 22卷 / 04期
关键词
D O I
10.1007/BF02386007
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A method is reported for in vivo bromodeoxyuridine incorporation in mice and its subsequent visualization in kidney by immunohistochemistry. Following formal-sublimate fixation of the kidney, bromodeoxyuridine labelled nuclei were detected in paraffin sections with a monoclonal antibody and visualized by an immunoperoxidase technique. This rapid and unequivocal method was used to measure labelling indices in tubules, glomerular tuft and Bowman's capsule in normal male T70 (Beige) mice, at intervals up to 72 h after labelling. Significant differences were found between the labelling indices of these three populations of cells, which appeared to show different cell kinetic behaviour. © 1990 Chapman and Hall Ltd.
引用
收藏
页码:209 / 214
页数:6
相关论文
共 19 条
[1]  
Bancroft J. D., 1982, THEORY PRACTICE HIST, V2
[2]   INFLUENCE OF FIXATION ON IMMUNOPEROXIDASE STAINING OF PLASMA-CELLS IN PARAFFIN SECTIONS OF INTESTINAL BIOPSY SPECIMENS [J].
BOSMAN, FT ;
LINDEMAN, J ;
KUIPER, G ;
VANDERWAL, A ;
KREUNIG, J .
HISTOCHEMISTRY, 1977, 53 (01) :57-62
[3]  
CAWOOD AH, 1983, CELL TISSUE KINET, V16, P51
[4]   EFFECTS OF FIXATION AND PROCESSING ON IMMUNOHISTOCHEMICAL DEMONSTRATION OF IMMUNOGLOBULIN IN PARAFFIN SECTIONS OF TONSIL AND BONE-MARROW [J].
CURRAN, RC ;
GREGORY, J .
JOURNAL OF CLINICAL PATHOLOGY, 1980, 33 (11) :1047-1057
[5]   INVIVO BROMODEOXYURIDINE INCORPORATION IN HUMAN GASTRIC-CANCER - A STUDY ON FORMALIN-FIXED AND PARAFFIN-EMBEDDED SECTIONS [J].
DANOVA, M ;
RICCARDI, A ;
BRUGNATELLI, S ;
FIOCCA, R ;
GIRINO, M ;
VILLANI, L ;
GIORDANO, P ;
DIONIGI, P ;
GIORDANO, M ;
BUTTINI, R ;
UCCI, G ;
MAZZINI, G .
HISTOCHEMICAL JOURNAL, 1988, 20 (03) :125-130
[6]   CELL-CYCLE ANALYSIS USING A MONOCLONAL-ANTIBODY TO BRDURD [J].
DEAN, PN ;
DOLBEARE, F ;
GRATZNER, H ;
RICE, GC ;
GRAY, JW .
CELL AND TISSUE KINETICS, 1984, 17 (04) :427-436
[7]   IMMUNOHISTOCHEMICAL DEMONSTRATION OF DNA-INCORPORATED 5-BROMODEOXYURIDINE IN FROZEN AND PLASTIC EMBEDDED SECTIONS [J].
HARMS, G ;
VANGOOR, H ;
KOUDSTAAL, J ;
DELEY, L ;
HARDONK, MJ .
HISTOCHEMISTRY, 1986, 85 (02) :139-143
[8]   SIMULTANEOUS IMMUNOHISTOCHEMICAL DEMONSTRATION OF ANTIGEN EXPRESSION AND 5-BROMODEOXYURIDINE INCORPORATION IN PLASTIC EMBEDDED SECTIONS [J].
HARMS, G ;
VANGOOR, H ;
KOUDSTAAL, J ;
DELEY, L ;
HARDONK, MJ .
HISTOCHEMISTRY, 1987, 86 (04) :393-395
[9]   EFFECTS OF FIXATION TIME AND ENZYMATIC DIGESTION ON IMMUNOHISTOCHEMICAL DEMONSTRATION OF BROMODEOXYURIDINE IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE [J].
HAYASHI, Y ;
KOIKE, M ;
MATSUTANI, M ;
HOSHINO, T .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1988, 36 (05) :511-514