ANALYSIS OF THE ANTIGENIC DIFFERENCE BETWEEN VERO-TOXIN-2 (VT2) AND VT2 VARIANT (VT2VH) OF VEROTOXIN-PRODUCING ESCHERICHIA-COLI BY A SITE-DIRECTED MUTAGENESIS

被引：8

作者：

ITO, H

NISHIBUCHI, M

TAKEDA, Y

机构：

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 79卷 / 01期

关键词：

VERO TOXIN; ANTIGENIC DIFFERENCE; SITE-DIRECTED MUTAGENESIS;

D O I：

10.1016/0378-1097(91)90521-B

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ouchterlony double gel diffusion analysis of the A and B subunits of purified Vero toxin 2 (VT2) and a variant of VT2 (VT2vh) demonstrated that the difference in antigenicity between VT2 and VT2vh is due to the difference in the B subunit of the two toxins. Analysis of mutants of VT2vh prepared by site-directed mutagenesis attributed the antigenic dissimilarity to the difference in the amino acid residue at position 24 of the B subunit.

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页码：27 / 30

页数：4

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