ANALYSIS OF MONOSACCHARIDE COMPOSITION OF MUCIN OLIGOSACCHARIDE-ALDITOLS BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY

被引：38

作者：

KARLSSON, NG ^{[1
]}

HANSSON, GC ^{[1
]}

机构：

[1] GOTHENBURG UNIV,DEPT MED BIOCHEM,S-41390 GOTHENBURG,SWEDEN

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 224卷 / 02期

关键词：

D O I：

10.1006/abio.1995.1084

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple one-step method for the analysis of monosaccharides including galactosaminitol after acidic hydrolysis is described. The hydrolyzate was re-N-acetylated and analyzed by high-performance anion-exchange chromatography and the sugars were detected by a pulsed amperometric detector. The method was applied on a mixture of neutral oligosaccharides released from mucin glycopeptides of rat small intestine by alkaline borohydride. Sugars were detected down to the nanomole range and the results were compared with monosaccharide compositional analysis performed by gas chromatography of acetylated alditols. (C) 1995 Academic Press, Inc.

引用

页码：538 / 541

页数：4

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