CLONING AND STRUCTURE OF THE GENE ENCODING THE HUMAN N-METHYL-D-ASPARTATE RECEPTOR (NMDAR1)

被引：33

作者：

ZIMMER, M

FINK, TM

FRANKE, Y

LICHTER, P

SPIESS, J

机构：

[1] MAX PLANCK INST EXPTL MED,DEPT MOLEC NEUROENDOCRINOL,D-37075 GOTTINGEN,GERMANY

[2] DEUTSCH KREBSFORSCHUNGSZENTRUM,SCHWERPUNKT ANGEW TUMORVIROL,D-69120 HEIDELBERG,GERMANY

来源：

GENE | 1995年 / 159卷 / 02期

关键词：

ALTERNATIVE SPLICING; GENOMIC SEQUENCING; GLUTAMATE RECEPTOR; HOMEOBOX PROTEIN; SUBTELOMERIC REPEAT;

D O I：

10.1016/0378-1119(95)00044-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The complete gene encoding the human N-methyl-D-aspartate receptor subunit NR1 (NMDAR1) has been isolated on a single cosmid clone, The gene is composed of 21 exons distributed over a total length of about 31 kb. More than 24 kb were sequenced, Exons 4, 20 and 21 are identical in their amino-acid sequence to those exons that are subject to alternative splicing in rat, indicating that all eight NMDAR1 isoforms found in rat will also be expressed in the human brain, Computer analysis of the pre-mRNA sequence revealed no secondary structures stable enough to explain alternative splicing, We suggest that cell-specific factors control expression of different isoforms. The promoter region contains two perfect copies of the recognition sequence for the Drosophila even-skipped protein, indicating that the developmentally regulated expression of NMDAR1 is controlled by a homeobox protein. The complete cosmid clone covering NMDAR1 was mapped to chromosome 9q34.3-qter by fluorescent in situ hybridisation (FISH). The telomeric location is supported by an imperfect (CA)(n) repeat homologous to a subtelomeric repeat on chromosome 16p.

引用

页码：219 / 223

页数：5

共 28 条

[1] CHROMOSOMAL ABERRATION AND SISTER-CHROMATID EXCHANGE FREQUENCIES IN PERIPHERAL-BLOOD LYMPHOCYTES OF A LARGE HUMAN-POPULATION SAMPLE .2. EXTENSION OF AGE RANGE [J].