ANCHORED PCR CLONING OF THE NOVEL HLA-CW-ASTERISK-0704 ALLELE DETECTED BY PCR-SSP

被引:10
作者
VILCHES, C [1 ]
BUNCE, M [1 ]
DEPABLO, R [1 ]
HERRERO, MJ [1 ]
KREISLER, M [1 ]
机构
[1] OXFORD TRANSPLANT CTR,TISSUE TYPING LAB,OXFORD,ENGLAND
来源
TISSUE ANTIGENS | 1995年 / 46卷 / 01期
关键词
PCR; CW-ASTERISK-0704; CW7; NEW HLA-C ALLELE; NUCLEOTIDE SEQUENCE; PCR-SSP; RACE;
D O I
10.1111/j.1399-0039.1995.tb02471.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The novel HLA-Cw*0704 allele, previously detected as the PCR-SSP variant Cw7/8v, has been cloned and sequenced from the homozygous typing cell KRO3/4 after amplification by anchored PCR. The nucleotide sequence of Cw*0704 is closely related to those of other Cw*07 alleles, but carries specific changes in exon 3 consistent with its serological behavior - a short Cw7 cross-reactive with antibodies directed against HLA-Cw8. Some of the substitutions of Cw*0704 have not been previously described for HLA-C but are found in HLA-B alleles and in published C sequences of non-human primates. The new allele carries a novel polymorphism in its 5' untranslated region (5'ut) that could be shared by all Cw*07 alleles. By PCR-SSP, Cw*0704 is a relatively common allele in English Caucasoids at a frequency of 4.6%. It is most often observed on HLA-B44 haplotypes previously described as HLA-C ''blank'', although linkage disequilibria with other HLA-B specificities have been found.
引用
收藏
页码:19 / 23
页数:5
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