Dielectric behavior of non-spherical cells in culture

被引:70
作者
Asami, K [1 ]
Yonezawa, T [1 ]
机构
[1] SUNTORY LTD,DIV PROD BEER 3,SHIMAMOTO,OSAKA 618,JAPAN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1245卷 / 03期
关键词
dielectric relaxation; permittivity; interfacial polarization; non-spherical cell; synchronized culture; growth curve;
D O I
10.1016/0304-4165(95)00116-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In order to study dielectric behavior of non-spherical cells growing in suspension culture, a dielectric theory has been developed based on the shell-ellipsoid model that is a conducting ellipsoid covered with a thin insulating shell. The theory predicts three dielectric relaxations for a suspension of ellipsoidal cells with three different semiaxes. For prolate spheroidal cells with two different semiaxes that show two dielectric relaxations the effect of the axial ratio on the dielectric relaxations was examined in detail. The low-frequency relaxation attributed to the component along the major axis strongly depends on the axial ratio, while the high-frequency relaxation due to the component along the minor axis is rather insensitive to the axial ratio. The theory is also applicable to simulation of dielectric behavior of yeast cells in synchronized and asynchronized culture by assuming that budding yeast cells are prolate spheroids.
引用
收藏
页码:317 / 324
页数:8
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