IMPROVED DETECTION OF HIV-1 ENVELOPE SEQUENCES USING OPTIMIZED PCR AND INOSINE-SUBSTITUTED PRIMERS

被引：21

作者：

CASSOL, S

SALAS, T

LAPOINTE, N

ARELLA, M

RUDNIK, J

OSHAUGHNESSY, M

机构：

[1] HOP ST JUSTINE, MONTREAL H3T 1C5, QUEBEC, CANADA

[2] UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH VIROL, LAVAL H7N 4Z3, QUEBEC, CANADA

来源：

MOLECULAR AND CELLULAR PROBES | 1991年 / 5卷 / 02期

关键词：

HIV-1; POLYMERASE CHAIN REACTION; INOSINE;

D O I：

10.1016/0890-8508(91)90011-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe inosine-substituted 'consensus' primers for the detection of HIV-1 envelope sequences by the polymerase chain reaction. The primers, modifications of SK68 and SK69, are highly specific (100%) and sensitive (> 94%) and they prevent false-negative results due to variation in the HIV-1 genome. Consensus primers are needed to ensure the detection of most, if not all, variants of HIV-1 including American, African and newly emerging strains. © 1991.

引用

页码：157 / 160

页数：4

共 6 条

[1] HIGHLY DEGENERATE, INOSINE-CONTAINING PRIMERS SPECIFICALLY AMPLIFY RARE CDNA USING THE POLYMERASE CHAIN-REACTION [J].