FIBEROPTIC BIOSENSOR FOR HYPOXANTHINE AND XANTHINE BASED ON A CHEMILUMINESCENCE REACTION

被引：49

作者：

HLAVAY, J ^{[1
]}

HAEMMERLI, SD ^{[1
]}

GUILBAULT, GG ^{[1
]}

机构：

[1] UNIV NEW ORLEANS,DEPT CHEM,NEW ORLEANS,LA 70148

来源：

BIOSENSORS & BIOELECTRONICS | 1994年 / 9卷 / 03期

基金：

匈牙利科学研究基金会;

关键词：

FIBEROPTIC BIOSENSOR; HYPOXANTHINE; XANTHINE; LUMINOL; CHEMILUMINESCENCE REACTION;

D O I：

10.1016/0956-5663(94)80121-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Fibre-optic biosensors were constructed for determination of hypoxanthine and xanthine. Xanthine oxidase and peroxidase were immobilized on different preactivated membranes which were subsequently mounted onto the tip of a fibre-optic bundle. The H2O2 generated by the reaction of hypoxanthine and xanthine oxidase was measured by chemiluminescence (CL) detection using luminol and peroxidase. A linear calibration curve of the sensors in the range of 1-316 muM hypoxanthine and 3.1-316 muM xanthine, respectively, with a detection limit of 0.55 muM hypoxanthine was obtained. Recovery of hypoxanthine ranged between 91 and 102%.

引用

页码：189 / 195

页数：7

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