PROTEIN-KINASES IN DIVERGENT EUKARYOTES - IDENTIFICATION OF PROTEIN-KINASE ACTIVITIES REGULATED DURING TRYPANOSOME DEVELOPMENT

被引:67
作者
PARSONS, M
VALENTINE, M
CARTER, V
机构
[1] UNIV WASHINGTON,DEPT PATHOBIOL,SEATTLE,WA 98195
[2] UNIV WASHINGTON,DEPT MICROBIOL,SEATTLE,WA 98195
关键词
GIARDIA-LAMBLIA; TRICHOMONAS-VAGINALIS; TYROSINE PHOSPHORYLATION;
D O I
10.1073/pnas.90.7.2656
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The role of protein kinases in organisms that diverged early in the eukaryotic lineage is relatively unexplored. In this study, we determined that primitive parasitic protozoa possess multiple protein-serine kinases and inferred the presence of protein-tyrosine kinases through sensitive immunoblotting techniques. To further explore the role of protein kinases in parasite development, we examined the activity of eight renaturable protein kinases during the life cycle of the protozoan parasite Trypanosoma brucei. The activities of six protein-serine/threonine kinases were regulated during development, with several distinct patterns of regulation. In addition, an 89-kDa protein kinase was detected in dividing cells but not in nondividing cells. Our data indicate that even the most primitive eukaryotes possess a large complement of protein kinases, including protein-tyrosine kinases as well as protein-serine/threonine kinases. The data further suggest that protein kinases may play a pivotal role in regulation of proliferation and differentiation in protozoa.
引用
收藏
页码:2656 / 2660
页数:5
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