INHIBITION OF HIV-1 REPLICATION BY A HIGH-COPY-NUMBER VECTOR EXPRESSING ANTISENSE RNA FOR REVERSE-TRANSCRIPTASE

被引：16

作者：

MEYER, J

NICK, S

STAMMINGER, T

GRUMMT, F

JAHN, G

LIPPS, HJ

机构：

[1] UNIV TUBINGEN,MED NAT WISSENSCH FORSCHUNGSZENTRUM,OB HIMMELREICH 7,W-7400 TUBINGEN 1,GERMANY

[2] UNIV ERLANGEN NURNBERG,INST KLIN & MOLEK VIROL,W-8520 ERLANGEN,GERMANY

[3] UNIV WURZBURG,BIOZENTRUM,INST BIOCHEM,W-8700 WURZBURG,GERMANY

来源：

GENE | 1993年 / 129卷 / 02期

关键词：

MAMMALIAN EXPRESSION VECTOR; GENE AMPLIFICATION; AIDS; T-LYMPHOCYTES; GENE THERAPY;

D O I：

10.1016/0378-1119(93)90277-A

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report the construction of a high-copy-number (hcn) expression vector for human cells. Amplification of this vector occurs due to the presence of an element derived from the murine DNA encoding ribosomal RNA (rDNA). HIV-1 replication in Jurkat T lymphocytes is nearly abolished when antisense RNA directed against the gene encoding reverse transcriptase is expressed from this hcn vector. The replication of the virus is only slightly reduced by the plasmid control version lacking the murine amplification-promoting element. This kind of hcn vector may represent an important improvement for the genetic engineering of eukaryotic cells and may also provide some ideas for the future gene therapy of some human diseases.

引用

页码：263 / 268

页数：6

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