PRENATAL-DIAGNOSIS OF HEMOGLOBINOPATHIES - A POTENTIAL APPLICATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：12

作者：

ALTER, BP ^{[1
]}

STUMP, DD ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED, DEPT PEDIAT, NEW YORK, NY 10029 USA

来源：

HEMOGLOBIN | 1987年 / 11卷 / 04期

关键词：

D O I：

10.3109/03630268709042853

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High performance liquid chromatography was compared with carboxymethyl cellulose column chromatography data for analysis of globin chain composition and biosynthetic ratios in fetal blood. A formula was derived for mathematical correction for losses of small amounts of globin protein on the high-performance liquid chromatography column. Radioactive globin chain ratios by high performance liquid chromatography are equivalent to those by carboxymethyl cellulose column chromatography, but only when carrier Hb A is added. We conclude that high performance liquid chromatography provides a suitable alternative to carboxymethyl cellulose columns, and has several advantages.

引用

页码：341 / 352

页数：12

共 13 条

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