CHARACTERIZATION OF THE CITRATE SYNTHASE REACTION WITH PROPIONYL-COA

被引:6
作者
BRANDANGE, S [1 ]
JOSEPHSON, S [1 ]
MAHLEN, A [1 ]
MORCH, L [1 ]
机构
[1] NATL BACTERIOL LAB, DEPT CHEM, S-10521 STOCKHOLM, SWEDEN
来源
ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY | 1984年 / 38卷 / 08期
关键词
D O I
10.3891/acta.chem.scand.38b-0695
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Experiments with propionyl-CoA stereoselectively deuterated in the propionyl moiety demonstrate that the formation of (2S,3S)-methylcitric acid (1) catalyzed by citrate (si)-synthase occurs with inversion of configuration in the propionyl moiety; the absolute configurations of 1 and (2S,3R)-methylcitronic acid indicate a si attack on oxaloacetate. Deuterium in the pro-S position is exchanged for protium 60 times faster than deuterium in the por-R position. Experiments with (R,S)-(2-2H1)propionyl-CoA allowed the determination of isotope effects. For the enzymatic formation of 1, a primary deuterium isotope effect kH/kD = 1.8 and a secondary .alpha.-deuterium isotope effect kH/KD = 0.99 were calculated; both are effects on Vmax/Km.
引用
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页码:695 / 700
页数:6
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