STABILITY OF ANTIBODY ATTACHMENT IN IMMUNOSORBENT CHROMATOGRAPHY

Detachment of immobilized antibody from its support matrix in an immunosorbent system prepared by the cyanogen bromide activation route was demonstrated. The antibody detachment from the support matrix occurred in mor than one antigen-antibody system suggesting that the leakage phenomenon may be a widespread disadvantage associated with the cyanogen bromide activation procedure. Detachment of the antibody could be reduced to less than 10 ng ml** minus **1 by immobilizing antibody on the properly oxidized polysaccharide support material or on the N-hydroxysuccinimide activated ester gel. Antibody dissociation from the matrix did not occur when antibodies were immobilized by either amine or amide attachment, thus, immunosorbents prepared by such strategies are suitable for the immunochromatographic purification of proteins from complex mixtures.