THE PROTEASOME MULTICATALYTIC MULTIFUNCTIONAL PROTEINASE - INVIVO FUNCTION IN THE UBIQUITIN-DEPENDENT N-END RULE PATHWAY OF PROTEIN-DEGRADATION IN EUKARYOTES

被引：72

作者：

RICHTERRUOFF, B ^{[1
]}

HEINEMEYER, W ^{[1
]}

WOLF, DH ^{[1
]}

机构：

[1] UNIV STUTTGART,INST BIOCHEM,PFAFFENWALDRING 55,W-7000 STUTTGART 80,GERMANY

来源：

FEBS LETTERS | 1992年 / 302卷 / 02期

关键词：

PROTEINASE YSCE; PROTEASOME MULTICATALYTIC MULTIFUNCTIONAL PROTEINASE; UBIQUITIN; N-END RULE; PROTEOLYSIS; YEAST;

D O I：

10.1016/0014-5793(92)80438-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteinase yscE, the proteasome/multicatalytic-multifunctional proteinase of yeast had been shown to function in stress response and in the degradation of ubiquitinated proteins [(1991) EMBO J. 10, 555-562]. A well-defined set of proteins degraded via ubiquitin-mediated proteolysis are the substrates of the N-end rule pathway [(1986) Science 234, 179-186; (1989) Science 243, 1576-1583]. We show that mutants defective in the chymotryptic activity of proteinase yscE fail to degrade substrates of the N-end rule pathway. This gives further proof of the proteasome being a central catalyst in ubiquitin-mediated proteolysis.

引用

页码：192 / 196

页数：5

共 35 条

[1]

ACHSTETTER T, 1984, J BIOL CHEM, V259, P3344

[2]

Achstetter T, 1985, Yeast, V1, P139, DOI 10.1002/yea.320010203

[3] A 20S PARTICLE UBIQUITOUS FROM YEAST TO HUMAN [J].

ARRIGO, AP ;

SIMON, M ;

DARLIX, JL ;

SPAHR, PF .

JOURNAL OF MOLECULAR EVOLUTION, 1987, 25 (02) :141-150

[4] IDENTITY OF THE 19S PROSOME PARTICLE WITH THE LARGE MULTIFUNCTIONAL PROTEASE COMPLEX OF MAMMALIAN-CELLS (THE PROTEASOME) [J].

ARRIGO, AP ;

TANAKA, K ;

GOLDBERG, AL ;

WELCH, WJ .

NATURE, 1988, 331 (6152) :192-194

[5]

Ausubel FM, 1987, CURRENT PROTOCOLS MO

[6] THE DEGRADATION SIGNAL IN A SHORT-LIVED PROTEIN [J].

BACHMAIR, A ;

VARSHAVSKY, A .

CELL, 1989, 56 (06) :1019-1032

[7] INVIVO HALF-LIFE OF A PROTEIN IS A FUNCTION OF ITS AMINO-TERMINAL RESIDUE [J].

BACHMAIR, A ;

FINLEY, D ;

VARSHAVSKY, A .

SCIENCE, 1986, 234 (4773) :179-186

[8] THE RECOGNITION COMPONENT OF THE N-END RULE PATHWAY [J].

BARTEL, B ;

WUNNING, I ;

VARSHAVSKY, A .

EMBO JOURNAL, 1990, 9 (10) :3179-3189

[9] A MULTIUBIQUITIN CHAIN IS CONFINED TO SPECIFIC LYSINE IN A TARGETED SHORT-LIVED PROTEIN [J].

CHAU, V ;

TOBIAS, JW ;

BACHMAIR, A ;

MARRIOTT, D ;

ECKER, DJ ;

GONDA, DK ;

VARSHAVSKY, A .

SCIENCE, 1989, 243 (4898) :1576-1583

[10] THE N-END RULE IS MEDIATED BY THE UBC2(RAD6) UBIQUITIN-CONJUGATING ENZYME [J].

DOHMEN, RJ ;

MADURA, K ;

BARTEL, B ;

VARSHAVSKY, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :7351-7355

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