STRUCTURE AND EXPRESSION OF THE GENE ENCODING RIBOSOMAL-PROTEIN-S1 FROM THE CYANOBACTERIUM SYNECHOCOCCUS SP STRAIN PCC-6301 - STRIKING SEQUENCE SIMILARITY TO THE CHLOROPLAST RIBOSOMAL PROTEIN-CS1

被引:19
作者
SUGITA, M [1 ]
SUGITA, C [1 ]
SUGIURA, M [1 ]
机构
[1] NAGOYA UNIV, CTR GENE RES, NAGOYA, AICHI 46401, JAPAN
来源
MOLECULAR AND GENERAL GENETICS | 1995年 / 246卷 / 02期
关键词
SSDNA-BINDING PROTEIN; CYANOBACTERIA; RIBOSOMAL PROTEIN S1; ENDOSYMBIOSIS;
D O I
10.1007/BF00294676
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We isolated a 38 kDa ssDNA-binding protein from the unicellular cyanobacterium Synechococcus sp. strain PCC 6301 and determined its N-terminal amino acid sequence. A genomic clone encoding the 38 kDa protein was isolated by using a degenerate oligonucleotide probe based on the amino acid sequence. The nucleotide sequence and predicted amino acid sequence revealed that the 38 kDa protein is 306 amino acids long and homologous to the nuclear-encoded 370 amino acid chloroplast ribosomal protein CS1 of spinach (48% identity), therefore identifying it as ribosomal protein (r-protein) S1. Cyanobacterial and chloroplast S1 proteins differ in size from Escherichia coli r-protein S1 (557 amino acids). This provides an additional evidence that cyanobacteria are closely related to chloroplasts. The Synechococcus gene rps1 encoding S1 is located 1.1 kb downstream from psbB, which encodes the photosystem II P680 chlorophyll a apoprotein. An open reading frame encoding a potential protein of 168 amino acids is present between psbB and rps1 and its deduced amino acid sequence is similar to that of E. coli hypothetical 17.2 kDa protein. Northern blot analysis showed that rps1 is transcribed as a monocistronic mRNA.
引用
收藏
页码:142 / 147
页数:6
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