YAN FUNCTIONS AS A GENERAL INHIBITOR OF DIFFERENTIATION AND IS NEGATIVELY REGULATED BY ACTIVATION OF THE RAS1/MAPK PATHWAY

被引：287

作者：

REBAY, I

RUBIN, GM

机构：

[1] Howard Hughes Medical Institute Department of Molecular, Cell Biology University of California, Berkeley, Berkeley

来源：

CELL | 1995年 / 81卷 / 06期

关键词：

D O I：

10.1016/0092-8674(95)90006-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Drosophila yan has been postulated to act as an antagonist of the proneural signal mediated by the sevenless/Ras1/MAPK pathway. We have mutagenized the eight MAPK phosphorylation consensus sites of yan and examined the effects of overexpressing the mutant protein in transgenic flies and transfected S2 cultured cells. Our results suggest that phosphorylation by MAPK affects the stability and subcellular localization of yan, resulting in rapid down-regulation of yan activity. Furthermore, MAPK-mediated down-regulation of yan function appears to be critical for the proper differentiation of both neuronal and nonneuronal tissues throughout development, suggesting that yan is an essential component of a general timing mechanism controlling the competence of a cell to respond to inductive signals.

引用

页码：857 / 866

页数：10

共 29 条

[1] PRIMARY STRUCTURE AND EXPRESSION OF A PRODUCT FROM CUT, A LOCUS INVOLVED IN SPECIFYING SENSORY ORGAN IDENTITY IN DROSOPHILA
BLOCHLINGER, K
BODMER, R
JACK, J
JAN, LY
JAN, YN
[J]. NATURE, 1988, 333 (6174) : 629 - 635
[2] NUCLEOTIDE-SEQUENCE AND STRUCTURE OF THE SEVENLESS GENE OF DROSOPHILA-MELANOGASTER
BOWTELL, DDL
SIMON, MA
RUBIN, GM
[J]. GENES & DEVELOPMENT, 1988, 2 (06) : 620 - 634
[3] BRAND AH, 1993, DEVELOPMENT, V118, P401
[4] THE ETS DOMAIN PROTEIN POINTED-P2 IS A TARGET OF MAP KINASE IN THE SEVENLESS SIGNAL-TRANSDUCTION PATHWAY
BRUNNER, D
DUCKER, K
OELLERS, N
HAFEN, E
SCHOLZ, H
KLAMBT, C
[J]. NATURE, 1994, 370 (6488) : 386 - 389
[5] PHOSPHORYLATION OF THE C-FOS TRANSREPRESSION DOMAIN BY MITOGEN-ACTIVATED PROTEIN-KINASE AND 90-KDA RIBOSOMAL S6 KINASE
CHEN, RH
ABATE, C
BLENIS, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (23) : 10952 - 10956
[6] CLARKLEWIS I, 1991, J BIOL CHEM, V266, P15180
[7] COMPLEX CELLULAR AND SUBCELLULAR REGULATION OF NOTCH EXPRESSION DURING EMBRYONIC AND IMAGINAL DEVELOPMENT OF DROSOPHILA - IMPLICATIONS FOR NOTCH FUNCTION
FEHON, RG
JOHANSEN, K
REBAY, I
ARTAVANISTSAKONAS, S
[J]. JOURNAL OF CELL BIOLOGY, 1991, 113 (03) : 657 - 669
[8] MOLECULAR-INTERACTIONS BETWEEN THE PROTEIN PRODUCTS OF THE NEUROGENIC LOCI NOTCH AND DELTA, 2 EGF-HOMOLOGOUS GENES IN DROSOPHILA
FEHON, RG
KOOH, PJ
REBAY, I
REGAN, CL
XU, T
MUSKAVITCH, MAT
ARTAVANISTSAKONAS, S
[J]. CELL, 1990, 61 (03) : 523 - 534
[9] SIGNALING BY THE SEVENLESS PROTEIN TYROSINE KINASE IS MIMICKED BY RAS1 ACTIVATION
FORTINI, ME
SIMON, MA
RUBIN, GM
[J]. NATURE, 1992, 355 (6360) : 559 - 561
[10] AN ACTIVATED NOTCH RECEPTOR BLOCKS CELL-FATE COMMITMENT IN THE DEVELOPING DROSOPHILA EYE
FORTINI, ME
REBAY, I
CARON, LA
ARTAVANISTSAKONAS, S
[J]. NATURE, 1993, 365 (6446) : 555 - 557

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