Mammalian phospholipase D: Activation by ammonium sulfate and nucleotides

被引：23

作者：

Nakamura, S ^{[1
]}

Shimooku, K ^{[1
]}

Akisue, T ^{[1
]}

Jinnai, H ^{[1
]}

Hitomi, T ^{[1
]}

Kiyohara, Y ^{[1
]}

Ogino, C ^{[1
]}

Yoshida, K ^{[1
]}

Nishizuka, Y ^{[1
]}

机构：

[1] KOBE UNIV,BIOSIGNAL RES CTR,KOBE,HYOGO 650,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 26期

关键词：

phosphatidylcholine; G protein;

D O I：

10.1073/pnas.92.26.12319

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Phospholipase D (PLD) associated with the rat kidney membrane was activated by guanine 5'-[gamma-thio] triphosphate and a cytosol fraction that contained ADP-ribosylation factor, When assayed by measuring the phosphatidyl transfer reaction to ethanol with exogenously added radioactive phosphatidylcholine as substrate, the PLD required a high concentration (1.6 M) of ammonium sulfate to exhibit high enzymatic activity. Other salts examined were far less effective or practically inactive, and this dramatic action of ammonium sulfate is not simply due to such high ionic strength. Addition of ATP but not of nonhydrolyzable ATP analogue adenosine 5'-[beta,gamma-imido] diphosphate further enhanced the PLD activation approximate to 2- to 3-fold. This enhancement by ATP needed cytosol, implying a role of protein phosphorylation. A survey of PLD activity in rat tissues revealed that, unlike in previous observations reported thus far, PLD was most abundant in membrane fractions of kidney, spleen, and liver in this order, and the enzymatic activity in brain and lung was low.

引用

页码：12319 / 12322

页数：4

共 26 条

[1] GRANULOCYTE PHOSPHOLIPASE-D IS ACTIVATED BY A GUANINE-NUCLEOTIDE DEPENDENT PROTEIN FACTOR [J].