STABLE EXPRESSION OF HUMAN CYTOCHROME-P450IA2 CDNA IN A HUMAN LYMPHOBLASTOID CELL-LINE - ROLE OF THE ENZYME IN THE METABOLIC-ACTIVATION OF AFLATOXIN-B1

被引：61

作者：

CRESPI, CL ^{[1
]}

STEIMEL, DT ^{[1
]}

AOYAMA, T ^{[1
]}

GELBOIN, HV ^{[1
]}

GONZALEZ, FJ ^{[1
]}

机构：

[1] NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20205

来源：

MOLECULAR CARCINOGENESIS | 1990年 / 3卷 / 01期

关键词：

Carcinogen metabolism; DNA binding; gene mutation; Key words; transfection;

D O I：

10.1002/mc.2940030104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A human lymphoblastoid cell line stably expressing a human cytochrome P4501A2 cDNA was developed. This recombinant cell line displayed P4501A2 protein and estradiol2‐hydroxylase activity, neither of which was detected in the parental cell line. The recombinant cell line was also approximately 1000‐fold more sensitive t o the cyto‐ toxicity and mutagenicity of the carcinogenic mycotoxin aflatoxin B1 than was the parent cell line. The increase in mutagenicity was supported by a corresponding increase in the level of aflatoxin B1 binding t o DNA in cells expressing P4501A2 relative to control cells. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：5 / 8

页数：4

共 16 条

[1] HUMAN CDNA-EXPRESSED CYTOCHROME-P450 IA2 - MUTAGEN ACTIVATION AND SUBSTRATE-SPECIFICITY [J].