HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF LANSOPRAZOLE AND ITS METABOLITES IN HUMAN SERUM AND URINE

被引：61

作者：

AOKI, I

OKUMURA, M

YASHIKI, T

机构：

[1] TAKEDA CHEM IND LTD,PHARMACEUT PROD RES LABS,YODOGAWA KU,OSAKA 532,JAPAN

[2] TAKEDA CHEM IND LTD,ANALYT LABS,OSAKA 532,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 571卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80457-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and sensitive high-performance liquid chromatographic method with ultraviolet detection is described for the simultaneous determination of lansoprazole and its metabolites in human serum and urine. The analytes in serum or urine were extracted with diethyl ether-dichloromethane (7:3, v/v) followed by evaporation, dissolution and injection into a reversed-phase column. The recoveries of authentic analytes added to serum at 0.05-2-mu-g/ml or to urine at 1-20-mu-g/ml were greater than 88%, with the coefficients of variation less than 7.1%. The minimum determinable concentrations of all analytes were 5 ng/ml in serum and 50 ng/ml in urine. The method was successfully applied to a pharmacokinetic study of lansoprazole in human.

引用

页码：283 / 290

页数：8