SKIN THE TAPEWORMS BEFORE YOU STAIN THEIR NERVOUS-SYSTEM - A NEW METHOD FOR WHOLE-MOUNT IMMUNOCYTOCHEMISTRY

被引：20

作者：

GUSTAFSSON, MKS

机构：

[1] Department of Biology, Abo Akademi University, Åbo, SF-20500

来源：

PARASITOLOGY RESEARCH | 1991年 / 77卷 / 06期

关键词：

D O I：

10.1007/BF00928419

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A new method for making whole-mount immunocytochemical preparations of thick-skinned tapeworms is described. The tough tegument of the worms, which is impermeable to primary and secondary antisera, is removed by placing live worms in distilled water for 5-8 h, during which period the tegument is shed. The worms are thereafter fixed and stained as whole specimens using fluorescence immunocytochemical techniques. Both the main nervous structures and the finest neuronal details are revealed by this technique. Several types of antisera combinations are used. Diphyllobothrium dendriticum and Eubothrium crassum served as model organisms in this study.

引用

页码：509 / 516

页数：8

共 14 条

[1]

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[10] THE SEROTONINERGIC, CHOLINERGIC AND PEPTIDERGIC COMPONENTS OF THE NERVOUS-SYSTEM IN THE MONOGENEAN PARASITE, DICLIDOPHORA-MERLANGI - A CYTOCHEMICAL STUDY [J].

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PARASITOLOGY, 1990, 100 :255-273

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