SKIN CYTOGENETIC ASSAY FOR THE DETECTION OF CLASTOGENS-CARCINOGENS TOPICALLY ADMINISTERED TO MICE

被引：5

作者：

BARALE, R ^{[1
]}

SCAPOLI, C ^{[1
]}

FALEZZA, A ^{[1
]}

VENTURA, L ^{[1
]}

BERNACCHI, F ^{[1
]}

LOPRIENO, N ^{[1
]}

BARRAI, I ^{[1
]}

机构：

[1] UNIV PISA,DIPARTIMENTO SCI AMBIENTE & TERR,I-56100 PISA,ITALY

来源：

MUTATION RESEARCH | 1992年 / 271卷 / 03期

关键词：

MOUSE; SKIN; BONE MARROW; CHROMOSOMAL ABERRATIONS; SISTER-CHROMATID EXCHANGE; URETHANE; DIMETHYLBENZ[A]ANTHRACENE;

D O I：

10.1016/0165-1161(92)90017-G

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A method for assessing the effect of clastogens on mouse skin epidermal cells was devised and applied. Toxic and mutagenic responses in epidermal cells were tested using two known mutagens and carcinogens, urethane (URE) and 7,12-dimethylbenz[a]anthracene (DMBA). Cell generation time, sister-chromatid exchanges (SCE) and chromosomal aberrations (CA) after topical and intraperitoneal (i.p.) treatment were measured in epidermal and bone marrow cells. After topical administration both tissues responded similarly, whereas after i.p. treatment skin cells were less responsive than bone marrow cells. However, the results indicate the validity of this new cytogenetic approach for the assessment of the genotoxicity of compounds applied directly to skin.

引用

页码：223 / 230

页数：8

共 27 条

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