HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC RETENTION BEHAVIOR OF RING-SUBSTITUTED ANILINE DERIVATIVES ON A POROUS GRAPHITIZED CARBON COLUMN

The retentions of sixteen ring-substituted aniline derivatives were determined on a graphitized carbon column using unbuffered acetonitrile water and methanol water eluent mixtures at various organic phase concentrations. Principal component analysis calculated from both thc correlation and covariance matrices was used to detect the similarities and dissimilarities between the retention behaviours of the aniline derivatives. Each aniline derivative showed narrow and symmetrical peaks in each eluent. The high percentage of variance explained in the first principal component suggests that the eluents have common elution characteristics. but according to the second principal component they showed slightly different selectivities. The retention of aniline derivatives is not governed by their lipopholicity and bulkiness. The data indicate that a mixed type of retention mechanism accounts for the retention.