CHARACTERIZATION OF FUNCTIONAL HUMAN ERYTHROCYTE-TYPE GLUCOSE TRANSPORTER (GLUT1) EXPRESSED IN INSECT CELLS USING A RECOMBINANT BACULOVIRUS

被引：18

作者：

YI, CK

CHARALAMBOUS, BM

EMERY, VC

BALDWIN, SA

机构：

[1] UNIV LONDON,ROYAL FREE HOSP,SCH MED,DEPT BIOCHEM,ROWLAND HILL ST,LONDON NW3 2PF,ENGLAND

[2] UNIV LONDON,ROYAL FREE HOSP,SCH MED,DIV COMMUNICABLE DIS,LONDON NW3 2PF,ENGLAND

[3] UNIV LONDON,ROYAL FREE HOSP,SCH MED,DEPT PROTEIN & MOLEC BIOL,LONDON NW3 2PF,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1992年 / 283卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/bj2830643

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human erythrocyte-type glucose transporter (GLUT1) has been abundantly expressed in insect cells by using a recombinant baculovirus. At 4 days after infection with the virus, the insect cell-surface and intracellular membranes were found to contain > 200 pmol of D-glucose-sensitive binding sites for the transport inhibitor cytochalasin B per mg of protein. The characteristics of binding were identical with those of the erythrocyte transporter, although the two proteins differed substantially in apparent M(r), probably as a result of glycosylation differences.

引用

页码：643 / 646

页数：4

共 18 条

[1] MONOSACCHARIDE TRANSPORTER OF THE HUMAN-ERYTHROCYTE - CHARACTERIZATION OF AN IMPROVED PREPARATION [J].