SYNTHESIS OF NOVEL FLUOROGENIC EDMAN REAGENTS, 7-N,N-DIMETHYLAMINOSULPHONYL-4-(2,1,3-BENZOXADIAZOLYL)ISOTHIOCYANATE (DBD-NCS) AND 7-AMINOSULPHONYL-4-(2,1,3-BENZOXADIAZOLYL)-ISOTHIOCYANATE (ABD-NCS)

被引：28

作者：

IMAI, K ^{[1
]}

UZU, S ^{[1
]}

NAKASHIMA, K ^{[1
]}

AKIYAMA, S ^{[1
]}

机构：

[1] NAGASAKI UNIV,SCH PHARMACEUT SCI,NAGASAKI 852,JAPAN

来源：

BIOMEDICAL CHROMATOGRAPHY | 1993年 / 7卷 / 01期

关键词：

D O I：

10.1002/bmc.1130070114

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel fluorogenic Edman reagents, 7-N,N-dimethylaminosulphonyl-4-(2.1,3-benzoxadiazolyl)isothiocyanate (DBD-NCS) and 7-aminosulphonyl-4-(2,1,3-benzoxadiazolyl)isothiocyanate (ABD-NCS) having no fluorescence themselves were synthesized. The derivatives of amino acids with DBD-NCS fluoresce at 505 nm with excitation at 385 nm. They were separated on a reversed-phase HPI.C column and detected at the sub-picomol level. Ala-Phe derivatized with DBD-NCS was cleaved by acid to generate DBD-thiocarbamyl-Ala.

引用

页码：56 / 57

页数：2

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