IN-VITRO CYTOKINE MODULATION OF INTERCELLULAR-ADHESION MOLECULE-1 EXPRESSION ON SYSTEMIC-SCLEROSIS NORMAL DERMAL FIBROBLASTS

被引:17
作者
CHO, MM
JIMENEZ, SA
JOHNSON, BA
HARLOW, LA
BURROWS, JC
KOCH, AE
机构
[1] THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, PHILADELPHIA, PA 19107 USA
[2] LAKESIDE VET ADM MED CTR, CHICAGO, IL USA
[3] NORTHWESTERN UNIV, SCH MED, DEPT MED, CHICAGO, IL 60611 USA
关键词
SYSTEMIC SCLEROSIS; CELL ADHESION; FIBROBLAST; SKIN; LEUKOCYTES;
D O I
10.1159/000163881
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Our objective was to study the regulation of intercellular adhesion molecule-1 (ICAM-1) expression by cytokines on cultured fibroblasts obtained from systemic sclerosis and normal skin. ICAM-1 expression on dermal fibroblasts obtained from diffuse systemic sclerosis patients with early disease(less than or equal to 2 years) and normal dermal fibroblasts incubated with and without cytokines was measured by radioimmunoassay and flow cytometry. Systemic sclerosis dermal fibroblasts expressed lower basal levels of ICAM-1 than did normal dermal fibroblasts. Both the normal and systemic sclerosis dermal fibroblasts increased their cell surface expression of ICAM-1 in response to interleukin-1 beta (IL 1 beta), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma(IFN-gamma) in a dose-dependent fashion. Systemic sclerosis dermal fibroblasts appeared to be hyperresponsive to IL-1 beta, TNF-alpha, and IFN-gamma. ICAM-1 expression in response to cytokine stimulation increased to a greater degree on systemic sclerosis compared to normal dermal fibroblasts. The enhanced ICAM-1 expression may play a role in the retention of leukocytes involved in systemic sclerosis skin lesions.
引用
收藏
页码:73 / 81
页数:9
相关论文
共 51 条
[1]   EXPRESSION AND FUNCTION OF SURFACE-ANTIGENS ON SCLERODERMA FIBROBLASTS [J].
ABRAHAM, D ;
LUPOLI, S ;
MCWHIRTER, A ;
PLATERZYBERK, C ;
PIELA, TH ;
KORN, JH ;
OLSEN, I ;
BLACK, C .
ARTHRITIS AND RHEUMATISM, 1991, 34 (09) :1164-1172
[2]   INTEGRINS AND OTHER CELL-ADHESION MOLECULES [J].
ALBELDA, SM ;
BUCK, CA .
FASEB JOURNAL, 1990, 4 (11) :2868-2880
[3]  
ALVAROGARCIA JM, 1990, ARTHRITUS RHEUM S, V33, P147
[4]   DIFFERENTIAL EXPRESSION OF ADHESION MOLECULES ON INFILTRATING CELLS IN INFLAMMATORY DERMATOSES [J].
BOEHNCKE, WH ;
KELLNER, I ;
KONTER, U ;
STERRY, W .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1992, 26 (06) :907-913
[5]  
BROUTYBOYE D, 1980, LYMPHOKINE REPORTS, V1, P99
[6]  
CARLOS T, 1990, IMMUNOL RES, V114, P128
[7]   ROLE OF CYTOKINES IN INFLAMMATORY SYNOVITIS - THE COORDINATE REGULATION OF INTERCELLULAR-ADHESION MOLECULE-1 AND HLA CLASS-I AND CLASS-II ANTIGENS IN RHEUMATOID SYNOVIAL FIBROBLASTS [J].
CHIN, JE ;
WINTERROWD, GE ;
KRZESICKI, RF ;
SANDERS, ME .
ARTHRITIS AND RHEUMATISM, 1990, 33 (12) :1776-1786
[8]  
CLAMAN HN, 1985, CELL IMMUNOL, V94, P73, DOI 10.1016/0008-8749(85)90086-3
[9]  
CLAMAN HN, 1991, ARTHRITIS RHEUM, V34, P1495
[10]   CYTOKINE REGULATION OF PROLIFERATION AND ICAM-1 EXPRESSION OF HUMAN DERMAL MICROVASCULAR ENDOTHELIAL-CELLS INVITRO [J].
DETMAR, M ;
TENORIO, S ;
HETTMANNSPERGER, U ;
RUSZCZAK, Z ;
ORFANOS, CE .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1992, 98 (02) :147-153