INCREASE OF GPC LEVELS IN CULTURED-MAMMALIAN-CELLS DURING ACIDOSIS - A P-31 MR SPECTROSCOPY STUDY USING A CONTINUOUS BIOREACTOR SYSTEM

The purpose of this study was to study the metabolic events during a slow acidosis in three different cell lines by combining P-31 magnetic resonance spectroscopy and hollow fiber bioreactor technology. The rate of change in intracellular pH, glycerophosphorylcholine (GPC), phophorylcholine (PCho), and nucleoside-triphosphate (NTP) levels were measured during 8 h of acidosis and 16 h of recovery in EPO, EAT, and RN1a cells, three cultured mammalians cell lines. Our results show a significant increase in GPC levels to 330 +/- 21, 540 +/- 25, and 220 +/- 21% of their initial value correlated to a decrease of PCho levels to 57 +/- 14, 58 +/- 17 and 45 +/- 15% of their initial value in EAT, RN1a, and EPO cells, respectively. These changes are discussed in terms of perturbation of energetic metabolism in cells undergoing a slow acidosis.