We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the Gag polyproteins tested, only those encoded by closely related retroviruses formed heteromultimers. To determine the primary sequence requirements for human immunodeficiency virus type 1 Gag polyprotein multimerization, we studied the effects on multimerization of deletion and linker insertion mutations. Sequences necessary for this process were located between the C-terminal one-third of the capsid domain and the C terminus of the nucleocapsid domain.
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PENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USA
BENNETT, RP
NELLE, TD
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PENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USA
NELLE, TD
WILLS, JW
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PENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USA
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PENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USA
BENNETT, RP
NELLE, TD
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h-index: 0
机构:
PENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USA
NELLE, TD
WILLS, JW
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h-index: 0
机构:
PENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR,SCH MED, DEPT MICROBIOL & IMMUNOL,POB 850, HERSHEY, PA 17033 USA