SPECIFICITY AND SEQUENCE REQUIREMENTS FOR INTERACTIONS BETWEEN VARIOUS RETROVIRAL GAG PROTEINS

被引:99
作者
FRANKE, EK
YUAN, HEH
BOSSOLT, KL
GOFF, SP
LUBAN, J
机构
[1] COLUMBIA UNIV,COLL PHYS & SURG,DEPT MED,NEW YORK,NY 10032
[2] COLUMBIA UNIV,COLL PHYS & SURG,HOWARD HUGHES MED INST,NEW YORK,NY 10032
[3] COLUMBIA UNIV,COLL PHYS & SURG,DEPT BIOCHEM & MOLEC BIOPHYS,NEW YORK,NY 10032
关键词
D O I
10.1128/JVI.68.8.5300-5305.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the Gag polyproteins tested, only those encoded by closely related retroviruses formed heteromultimers. To determine the primary sequence requirements for human immunodeficiency virus type 1 Gag polyprotein multimerization, we studied the effects on multimerization of deletion and linker insertion mutations. Sequences necessary for this process were located between the C-terminal one-third of the capsid domain and the C terminus of the nucleocapsid domain.
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页码:5300 / 5305
页数:6
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