MICROTUBULE-ASSOCIATED PROTEIN-1A IS THE FIBROBLAST HMW MAP UNDERGOING MITOGEN-STIMULATED SERINE PHOSPHORYLATION

被引：9

作者：

ERICKSON, AK

RAY, LB

STURGILL, TW

机构：

[1] UNIV VIRGINIA,SCH MED,DEPT MED,CHARLOTTESVILLE,VA 22908

[2] UNIV VIRGINIA,SCH MED,DEPT PHARMACOL,CHARLOTTESVILLE,VA 22908

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90884-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A variety of antibodies to microtubule-associated protein (MAP) have been used to demonstrate that phosphorylation of a 350 kDa microtubule-associated protein is stimulated 2-3 fold by epidermal growth factor or serum in quiescent 3T3-L1 fibroblasts and by insulin in 3T3-L1 adipocytes. Phosphorylation occurs on serine residues, and is maximal by 15-20 min. The phosphoprotein has been identified as MAP1A by specific immunoprecipitation with a well-characterized monoclonal antibody. © 1990.

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页码：827 / 832

页数：6

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