IDENTIFICATION OF DYSTROPHIN IN CARDIAC SARCOLEMMAL VESICLES

被引：7

作者：

MICHALAK, M

ZUBRZYCKAGAARN, E

机构：

[1] UNIV ALBERTA, DEPT PEDIAT, CARDIOVASC DIS RES GRP, 417 HERITAGE MED RES CTR, EDMONTON T6G 2S2, ALBERTA, CANADA

[2] UNIV ALBERTA, DEPT BIOCHEM, EDMONTON T6G 2S2, ALBERTA, CANADA

[3] HOSP SICK CHILDREN, RES INST, DEPT GENET, TORONTO M5G 1X8, ONTARIO, CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90368-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified dystrophin in highly purified sarcolemmal vesicles isolated from canine and bovine hearts using specific antibodies against the COOH-terminal region of the protein. Bovine cardiac sarcolemma contained a single immunoreactive protein band (Mr. ∼400,000) whereas the canine cardiac membrane contained a doublet (Mr. ∼420,000 and ∼380,000). The higher molecular weight form of canine cardiac dystrophin was more abundant than the lower molecular weight form. These highly purified preparations of the sarcolemmal vesicles should provide a useful tool for structural and functional analysis of the interaction of dystrophin with the plasma membrane. © 1990.

引用

页码：565 / 570

页数：6

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