IMMOBILIZED POLYMERIC MICROSPHERES - POLYACROLEIN MICROSPHERES COVALENTLY BOUND IN A MONOLAYER STRUCTURE ONTO GLASS SURFACES

被引:10
作者
MARGEL, S
DOLITZKY, Y
SIVAN, O
机构
[1] Department of Chemistry, Bar-Ilan University, Ramat-Gan
来源
COLLOIDS AND SURFACES | 1992年 / 62卷 / 03期
关键词
LATEXES; IMMOBILIZED MICROSPHERES; MICROSPHERES; POLYACROLEIN MICROSPHERES;
D O I
10.1016/0166-6622(92)80004-L
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Novel materials composed of an unpacked monolayer of polyacrolein microspheres covalently bonded onto glass surfaces have been prepared and characterized. These surfaces were synthesized by the covalent binding of long- and short-chain length omega-cyano-alkylsilane compounds (SiCl3(CH2)nCN and Si(OMe)3(CH2)nCN, where n = 3 and 16) onto glass substrates. The nitrile groups at the omega position were then reduced to primary amino groups. Polyacrolein microspheres of 0.7 mu-m average diameter were then bonded to the modified surfaces. This binding of the microspheres to the derivatized surfaces is achieved via polyvalent Schiff-base bonds formed by the interaction between aldehyde groups of the microspheres and omega-primary amino groups of the derivatized glass surfaces. The percent coverage of the glass surfaces by the microspheres was higher for surfaces coated with the trichloroalkylsilane compounds compared to trimethoxyalkylsilane compounds, and higher also for surfaces coated with the short-chain length alkylsilane compounds compared to surfaces coated with the long ones. The residual aldehyde groups of the immobilized microspheres can then be used for the covalent binding of amino ligands, e.g. protiens, in a single step and at physiological pH (or any other desired pH). The binding kinetics of bungarotoxin to the immobilized microspheres has been investigated. Fourier transform-infrared/attenuated total reflection, electron spectroscopy for chemical analysis, contact angle measurements and scanning electron microscopy have been used for the characterization of the alkylsilane coatings and the binding of the microspheres to these coatings.
引用
收藏
页码:215 / 230
页数:16
相关论文
共 48 条
[1]   MONOLAYER TRANSFORMATION BY NUCLEOPHILIC-SUBSTITUTION - APPLICATIONS TO THE CREATION OF NEW MONOLAYER ASSEMBLIES [J].
BALACHANDER, N ;
SUKENIK, CN .
LANGMUIR, 1990, 6 (11) :1621-1627
[2]   FUNCTIONALIZED SILOXY-ANCHORED MONOLAYERS WITH EXPOSED AMINO, AZIDO, BROMO, OR CYANO GROUPS [J].
BALACHANDER, N ;
SUKENIK, CN .
TETRAHEDRON LETTERS, 1988, 29 (44) :5593-5594
[3]   SYNTHESIS OF FATTY-ACIDS USING ORGANOCOPPER(I) ATE COMPLEXES DERIVED FROM GRIGNARD-REAGENTS [J].
BERGBREITER, DE ;
WHITESIDES, GM .
JOURNAL OF ORGANIC CHEMISTRY, 1975, 40 (06) :779-782
[4]   COMPARISON OF DIFFERENT MODELS FOR THE TESTING OF PILOCARPINE EYEDROPS USING CONVENTIONAL EYEDROPS AND A NOVEL DEPOT FORMULATION (NANOPARTICLES) [J].
DIEPOLD, R ;
KREUTER, J ;
HIMBER, J ;
GURNY, R ;
LEE, VHL ;
ROBINSON, JR ;
SAETTONE, MF ;
SCHNAUDIGEL, OE .
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 1989, 227 (02) :188-193
[5]  
FOWKES FM, 1964, ADV CHEM AM CHEM SOC, V43
[6]  
Gaines G. L., 1966, INSOLUBLE MONOLAYERS
[7]   RECENT DEVELOPMENTS IN AFFINITY-CHROMATOGRAPHY SUPPORTS [J].
GROMAN, EV ;
WILCHEK, M .
TRENDS IN BIOTECHNOLOGY, 1987, 5 (08) :220-224
[8]   ACID-BASE BEHAVIOR OF CARBOXYLIC-ACID GROUPS COVALENTLY ATTACHED AT THE SURFACE OF POLYETHYLENE - THE USEFULNESS OF CONTACT-ANGLE IN FOLLOWING THE IONIZATION OF SURFACE FUNCTIONALITY [J].
HOLMESFARLEY, SR ;
REAMEY, RH ;
MCCARTHY, TJ ;
DEUTCH, J ;
WHITESIDES, GM .
LANGMUIR, 1985, 1 (06) :725-740
[9]   SULFONATED LATEX PARTICLE AS ACID CATALYSTS FOR THE CONTINUOUS INVERSION OF SUCROSE [J].
KIM, JH ;
ELAASSER, MS ;
KLEIN, A ;
VANDERHOFF, JW .
JOURNAL OF APPLIED POLYMER SCIENCE, 1988, 35 (08) :2117-2131
[10]   POSSIBILITIES OF USING NANOPARTICLES AS CARRIERS FOR DRUGS AND VACCINES [J].
KREUTER, J .
JOURNAL OF MICROENCAPSULATION, 1988, 5 (02) :115-127