FLUORESCENCE-BASED ENZYMATIC ASSAY BY CAPILLARY ELECTROPHORESIS LASER-INDUCED FLUORESCENCE DETECTION FOR THE DETERMINATION OF A FEW BETA-GALACTOSIDASE MOLECULES

被引：45

作者：

CRAIG, D ^{[1
]}

ARRIAGA, EA ^{[1
]}

BANKS, P ^{[1
]}

ZHANG, Y ^{[1
]}

RENBORG, A ^{[1
]}

PALCIC, MM ^{[1
]}

DOVICHI, NJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON,AB T6G 2G2,CANADA

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 226卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1202

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-Galactosidase can be assayed by monitoring the generation of the fluorescent products, fluorescein-mono-beta-D-galactopyranoside and fluorescein, when the fluorogenic substrate fluorescein-di-beta-D-galactopyranoside is used. We have used capillary electrophoresis with ultrasensitive laser-induced fluorescence detection to monitor the formation of the fluorescent products of off-column enzymatic reactions. By analyzing as little as 40 pl of the enzymatic mixture we obtain limits of detection of 6.5 X 10(-14) M beta-galactosidase or 1.6 molecules based on the detection of fluorescein-mono-beta-D-galactopyranoside. (C) 1995 Academic Press, Inc.

引用

页码：147 / 153

页数：7

共 23 条

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