ISOLATION OF BOVINE EOSINOPHILS AND CHARACTERIZATION OF THEIR LEUKOTRIENE FORMATION

被引：3

作者：

FREIBURGHAUS, J ^{[1
]}

JORG, A ^{[1
]}

机构：

[1] UNIV FRIBOURG,INST BIOCHEM,DEPT BIOCHEM,RUE MUSEE 5,CH-1700 FRIBOURG,SWITZERLAND

来源：

AGENTS AND ACTIONS | 1990年 / 31卷 / 1-2期

关键词：

D O I：

10.1007/BF02003216

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method for the isolation of eosinophils from bovine blood is described. Eosinophils were prepurified in a simple centrifugation step and subsequently separated on a Percoll gradient into two populations with densities of 1.088 and 1095 g/cm3, respectively, and a purity of greater than 90%. No apparent differences could be observed in electron micrographs and most of the granules appeared homogenous. A minor fraction of granules appeared to have a lamellated crystalline core structure. Eosinophils of bovine blood generate leukotriene B4 and its stereoisomers but not sulfidopeptide leukotrienes (slow-reacting substance) when stimulated by the Ca-ionophore A23187. © 1990 Birkhäuser Verlag.

引用

页码：16 / 22

页数：7

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