MICROTUBULE-ASSOCIATED PROTEIN 1B (MAP1B) PROMOTES EFFICIENT TUBULIN POLYMERIZATION IN-VITRO

被引：35

作者：

PEDROTTI, B ^{[1
]}

ISLAM, K ^{[1
]}

机构：

[1] MARION MERRELL DOW RES INST,LEPETIT RES CTR,I-21040 GERENZANO,ITALY

来源：

FEBS LETTERS | 1995年 / 371卷 / 01期

关键词：

MAP1B; HIGH-MOLECULAR WEIGHT MAPS; TUBULIN; CYTOSKELETON; MICROTUBULE;

D O I：

10.1016/0014-5793(95)00842-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of MAP1B on tubulin polymerisation has been examined in reconstitution experiments using purified tubulin and MAP1B. Under the assembly conditions used, tubulin alone was incapable of polymerising, but addition of MAP1B resulted in rapid assembly into microtubules. The kinetics of MAP1B-promoted microtubule assembly examined using pseudo-first-order plots show that assembly is described by a single reaction rate, The calculated association rate constant for MAP1B was about 200 x 10(6) M(-1). s(-1) and this constant was one order of magnitude higher when compared with that for MAP2-promoted assembly.

引用

页码：29 / 31

页数：3

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