MONOCLONAL-ANTIBODIES DEFINE 8 INDEPENDENT ANTIGENIC REGIONS ON THE BOVINE LEUKEMIA-VIRUS (BLV) ENVELOPE GLYCOPROTEIN-GP51

被引：126

作者：

BRUCK, C ^{[1
]}

MATHOT, S ^{[1
]}

PORTETELLE, D ^{[1
]}

BERTE, C ^{[1
]}

FRANSSEN, JD ^{[1
]}

HERION, P ^{[1
]}

BURNY, A ^{[1
]}

机构：

[1] UNIV BRUSSELS, DEPT MOLEC BIOL, B-1640 RHODE ST GENESE, BELGIUM

来源：

VIROLOGY | 1982年 / 122卷 / 02期

关键词：

D O I：

10.1016/0042-6822(82)90234-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Monoclonal anti-BLV [bovine leukemia virus] gp51 antibodies (C15) are characterized. Competition antibody binding assays show that they are directed against 8 independent antigenic regions on the BLV gp51 molecule. Conformation or accessibility of some of these gp51 epitopes change with the test system used, namely the liquid phase radioimmunoassay with radiolabeled antigen or the solid phase enzyme immunoassay with plastic bound gp51 or BLV particles. A 2-site immunometric assay using monoclonal antibodies directed against 2 independent epitopes allows detection of isolated gp51 molecules at a minimal concentration of 0.4 ng/ml and is also suitable for the detection of BLV particles.

引用

页码：342 / 352

页数：11

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