SHOOT REGENERATION FROM STEM AND LEAF EXPLANTS OF DENDRANTHEMA-GRANDIFLORA TZVELEV (SYN CHRYSANTHEMUM-MORIFOLIUM RAMAT)

被引：56

作者：

KAUL, V ^{[1
]}

MILLER, RM ^{[1
]}

HUTCHINSON, JF ^{[1
]}

RICHARDS, D ^{[1
]}

机构：

[1] HORT RES INST KNOXFIELD,DEPT AGR & RURAL AFFAIRS,POB 174,FERNTREE GULLY,VIC 3156,AUSTRALIA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 21卷 / 01期

关键词：

chrysanthemum; regeneration; tissue culture;

D O I：

10.1007/BF00034487

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Adventitious shoots were regenerated from leaf and stem explants of eleven chrysanthemum cultivars. The optimum medium for both explant types contained Murashige & Skoog basal medium supplemented with 5 μM 6-benzylaminopurine and 5 μM α-napthaleneacetic acid. Generally, stem explants were superior to leaf explants. There were large cultivar differences in shoot regeneration frequency with three cultivars failing to respond over a wide range of hormone combinations. Shoots on stem explants appeared mainly to originate from cortical cells which rapidly divided and ruptured the epidermis. Regenerated shoots could be easily rooted, transferred to glasshouse conditions, and grown to flowering. All regenerated plants had the same morphological characteristics compared to plants derived from nodes. © 1990 Kluwer Academic Publishers.

引用

页码：21 / 30

页数：10

共 21 条

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