DNA STRAND EXCHANGE MEDIATED BY A RAD51-SSDNA NUCLEOPROTEIN FILAMENT WITH POLARITY OPPOSITE TO THAT OF RECA

被引：428

作者：

SUNG, P ^{[1
]}

ROBBERSON, DL ^{[1
]}

机构：

[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT MOLEC GENET,HOUSTON,TX 77030

来源：

CELL | 1995年 / 82卷 / 03期

关键词：

D O I：

10.1016/0092-8674(95)90434-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast RAD51 gene functions in genetic recombination and DNA double-strand break repair. In vitro, in the presence of ATP and replication protein A, RAD51 protein pairs single-stranded DNA (ssDNA) with homologous double-stranded DNA (dsDNA) and catalyzes strand exchange between the synapsed DNA partners. Electron microscopic analyses show that RAD51 forms helical filaments on both ssDNA and dsDNA, in which the DNA is highly extended. However, results presented here indicate that only the RAD51-ssDNA nucleoprotein filament is functionally relevant. Strand exchange is arrested when heterology is encountered in the duplex partner, and analysis of the configuration of the terminal joint thus formed reveals that pairing and strand exchange initiate at the 5' end of the complementary strand in the linear duplex, a reaction polarity opposite to that of the bacterial prototype RecA.

引用

页码：453 / 461

页数：9

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