POLYMORPHIC SSR (SIMPLE-SEQUENCE-REPEAT) MARKERS FOR CHROMOSOME-20

被引：26

作者：

MELIS, R ^{[1
]}

BRADLEY, P ^{[1
]}

ELSNER, T ^{[1
]}

ROBERTSON, M ^{[1
]}

LAWRENCE, E ^{[1
]}

GERKEN, S ^{[1
]}

ALBERTSEN, H ^{[1
]}

WHITE, R ^{[1
]}

机构：

[1] UNIV UTAH,HOWARD HUGHES MED INST,SALT LAKE CITY,UT 84112

来源：

GENOMICS | 1993年 / 16卷 / 01期

关键词：

D O I：

10.1006/geno.1993.1140

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chromosome 20-specific simple-sequence-repeat (SSR) markers were developed from a flow-sorted phage library (LL20NSOl), subcloned in Bluescript, and screened with a tetranucleotide repeat, (AAAG)6, to identify potentially polymorphic loci. Of 100 clones sequenced, 39 were selected to construct primers. Of these 39, 22 were polymorphic. Reference to the CEPH linkage database (version 5) permitted genetic mapping of 16 of the new markers to specific regions of chromosome 20. Ten of the SSRs showed heterozygosity indices (above 70%) that would qualify them as potential index markers. © 1993 Academic Press, Inc.

引用

页码：56 / 62

页数：7

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