THE 5'-LEADER SEQUENCE OF TOBACCO MOSAIC-VIRUS RNA MEDIATES INITIATION-FACTOR-4E-INDEPENDENT, BUT STILL INITIATION-FACTOR-4A-DEPENDENT TRANSLATION IN YEAST EXTRACTS

被引：40

作者：

ALTMANN, M

BLUM, S

WILSON, TMA

TRACHSEL, H

机构：

[1] UNIV BERN,INST BIOCHEM & MOLEK BIOL,BUHLSTR 28,CH-3012 BERN,SWITZERLAND

[2] RUTGERS STATE UNIV,COOK COLL,CTR AGR MOLEC BIOL,NEW BRUNSWICK,NJ 08903

来源：

GENE | 1990年 / 91卷 / 01期

关键词：

cell-free system; Gene expression; initiation factor-dependent extract; mRNA leader sequence; recombinant DNA; Saccharomyces cerevisiae; translation initiation;

D O I：

10.1016/0378-1119(90)90173-O

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Messenger RNAs encoding chloramphenicol acetyltransferase (CAT) with or without the 5′-leader sequence of tobacco mosaic virus (TMV) RNA were synthesized in vitro and translated in Saccharomyces cerevisiae extracts dependent on eukaryotic initiation factors eIF-4E or eIF-4A. The 5′-leader sequence of TMV RNA renders translation of CAT mRNA eIF-4E-independent but still 4A-dependent. © 1990.

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页码：127 / 129

页数：3

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