ACTIVATION OF HUMAN NATURAL-KILLER-CELLS BY THE PROTEIN-BOUND POLYSACCHARIDE PSK INDEPENDENTLY OF INTERFERON AND INTERLEUKIN-2

The protein-bound polysaccharide PSK was tested for the ability to activate human natural killer (NK) cells. When blood lymphocytes and purified CD3- CD16+ large granular lymphocytes (LGL) were treated in vitro overnight with PSK, they demonstrated enhanced NK cell activity against K562. The PSK-activated killer cells also lysed NK-resistant targets and freshly isolated autologous and allogeneic tumor cells. The PSK effect was observed with concentrations that could be obtained in the blood of cancer patients receiving oral administration of PSK. PSK-induced enhancement of NK activity was not abrogated by monoclonal antibodies (mAb) that neutralized interferon (IFN)alpha, IFN-gamma, or interleukin-2 (IL-2). In addition, mAb reactive with p55 (alpha-chain) or p75 (beta-chain) glycoproteins of IL-2 receptors had no effects on PSK-enhanced NK activity even when used simultaneously. These results indicate that the PSK could activate human NK cells independently of IFN and IL-2/IL-2R systems.