THE USE OF SYNTHETIC PEPTIDE COMBINATORIAL LIBRARIES FOR THE DETERMINATION OF PEPTIDE LIGANDS IN RADIO-RECEPTOR ASSAYS - OPIOID-PEPTIDES

被引：50

作者：

HOUGHTEN, RA

DOOLEY, CT

机构：

[1] Torrey Pines Institute for Molecular Studies, San Diego, CA 92121

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1993年 / 3卷 / 03期

关键词：

D O I：

10.1016/S0960-894X(01)80222-5

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A synthetic peptide combinatorial library (SPCL) was prepared, composed of 52,128,400 L-amino acid hexapeptides, which was used with an iterative selection process to determine peptides capable of inhibiting binding of [H-3] [D-Ala2,MePhe4,Gly-ol5]enkephalin (DAGO) to crude rat brain homogenates. The first five residues corresponded exactly to the naturally occurring opioid peptide sequences of methionine and leucine enkephalin.

引用

页码：405 / 412

页数：8

共 19 条

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