BETA-4 NTEGRIN EXPRESSION ON INVITRO AND INVIVO METASTATIC VARIANTS OF LEWIS LUNG-CARCINOMA

被引：3

作者：

CIMINO, L

PERROTTI, D

FALCIONI, R

KENNEL, SJ

SACCHI, A

机构：

[1] Molecular Oncogenesis Laboratory, Regina Elena Cancer Institute, Rome, 291, Viale Regina Elena

[2] Oak Ridge National Laboratory, Biology Division, 37830, Tennessee

来源：

CYTOTECHNOLOGY | 1991年 / 5卷

关键词：

D O I：

10.1007/BF00736805

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The expression of beta-4, alpha-6, and beta-1 integrin subunits has been investigated on some in vitro and in vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of alpha-6, beta-1, and beta-4 subunits we demonstrate that alpha-6 and beta-1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas beta-4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with beta-1 and beta-4 subunits demonstrate that a) significant amounts of beta-1 mRNA were detected in all metastatic variants of 3LL; b) mRNA corresponding to the described entire coding sequence of beta-4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that beta-4 subunit is specifically expressed in highly metastasizing cells of 3LL while is undetectable in lower metastasizing ones.

引用

页码：45 / 48

页数：4

共 12 条

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