GENOMIC ORGANIZATION AND ANALYSIS OF THE 5' END OF THE PORCINE RYANODINE RECEPTOR GENE (RYRL)

被引：31

作者：

BRENIG, B ^{[1
]}

BREM, G ^{[1
]}

机构：

[1] UNIV MUNICH,FAK VET MED,LEHRSTUHL MOLEK TIERZUCHT,W-8000 MUNICH 33,GERMANY

来源：

FEBS LETTERS | 1992年 / 298卷 / 2-3期

关键词：

RYANODINE RECEPTOR; MALIGNANT HYPERTHERMIA; STRESS SYNDROME; HALOTHANE; VOLATILE ANESTHETICS; SUS SCROFA-DOMESTICA;

D O I：

10.1016/0014-5793(92)80076-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study we describe the isolation of genomic clones of the 5' region of the porcine ryanodine receptor gene, a candidate for malignant hyperthermia in pigs and humans. The recombinants were isolated from a porcine liver, genomic DNA library in phage EMBL3A after screening with PCR amplified DNA fragments. The exon/intron structure of the ryanodine receptor gene was determined by DNA sequencing. Based on the sequence data it was possible to develop a simple test for the detection of malignant hyperthermia susceptible and normal pigs.

引用

页码：277 / 279

页数：3

共 23 条

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