APPLICATION OF HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS TO THE ANALYSIS OF H1 HISTONES

被引：32

作者：

LINDNER, H

WURM, M

DIRSCHLMAYER, A

SARG, B

HELLIGER, W

机构：

[1] Institute of Medical Chemistry and Biochemistry, Innsbruck

来源：

ELECTROPHORESIS | 1993年 / 14卷 / 5-6期

关键词：

D O I：

10.1002/elps.1150140174

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-performance capillary electrophoresis for the separation of rat testis H1 histone variants and their phosphorylated modifications is described. The influence of buffer pH, hydroxypropylmethyl cellulose, and buffer concentration has been investigated. Under optimized conditions (500 mM phosphate buffer, pH 2, 0.03% hydroxypropylmethyl cellulose) using an uncoated capillary, eight H1 histone subfractions, including two H1-degrees histones and H1t and their phosphorylated modifications, are resolved. Application of capillary electrophoresis to the separation of H1 histones provides an important new alternative to high-performance liquid chromatography (HPLC) and traditional gel electrophoresis.

引用

页码：480 / 485

页数：6

共 30 条

[1] STUDIES ON ROLE AND MODE OF OPERATION OF VERY LYSINE-RICH HISTONE H1 (F1) IN EUKARYOTE CHROMATIN - PROPERTIES OF N-TERMINAL AND C-TERMINAL HALVES OF HISTONE H1 [J].