RESTRICTION ENZYME FRAGMENT LENGTH POLYMORPHISMS OF AMPLIFIED HERPES-SIMPLEX VIRUS TYPE-1 DNA PROVIDE EPIDEMIOLOGIC INFORMATION

被引：9

作者：

HAUGEN, TH ^{[1
]}

ALDEN, B ^{[1
]}

MATTHEY, S ^{[1
]}

NICHOLSON, D ^{[1
]}

机构：

[1] VET AFFAIRS MED CTR,IOWA CITY,IA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 1993年 / 17卷 / 02期

关键词：

D O I：

10.1016/0732-8893(93)90023-Z

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Human herpes simplex type 1 (HSV-1) DNA of isolates from patients in a large teaching hospital was amplified by the polymerase chain reaction (PCR). The PCR products targeted approximately 2100 nt regions of relatively low G + C content. Comparison of restriction enzyme digests of amplified DNA showed variation useful for strain differentiation. Twelve nonrelated HSV-1 were differentiated from one another. In contrast, specimens epidemiologically related in an outbreak were indistinguishable from each other. Restriction endonuclease analysis of amplified HSV-1 sequences appears to be useful for molecular epidemiology and laboratory quality control to detect possible contamination by PCR products.

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页码：129 / 133

页数：5

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