AN IMPROVED METHYLATION PROCEDURE FOR THE ANALYSIS OF COMPLEX POLYSACCHARIDES INCLUDING RESISTANT STARCH AND A CRITIQUE OF THE FACTORS WHICH LEAD TO UNDERMETHYLATION

被引:60
作者
NEEDS, PW
SELVENDRAN, RR
机构
[1] Department of Food Molecular Biochemistry, AFRC Institute of Food Research, Norwich Laboratory, Norfolk, NR4 7UA, Norwich Research Park, Colney, Norwich
关键词
METHYLATION ANALYSIS; POLYSACCHARIDES; SODIUM HYDROXIDE; DIMETHYL SULFOXIDE; NONOXIDATIVE; RESISTANT STARCH;
D O I
10.1002/pca.2800040504
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Deficiencies in the widely used Hakomori method of methylation are described. Evidence is given that this method of linkage analysis often gives levels of undermethylation which hamper the detailed study of complex carbohydrates from plant cell walls. A modified method using sodium hydroxide as base is described which is capable of fully methylating polymers for which the Hakomori method generally fails and which does not promote the carbohydrate oxidation typical of a former procedure using this reagent. An explanation of its efficacy is advanced. Analyses of retrograded, alpha-amylase-resistant starches obtained by the modified method are reported.
引用
收藏
页码:210 / 216
页数:7
相关论文
共 41 条
[1]   RAPESEED HULL PECTIN [J].
ASPINALL, GO ;
JIANG, KS .
CARBOHYDRATE RESEARCH, 1974, 38 (01) :247-255
[2]   GAS-LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY IN METHYLATION ANALYSIS OF POLYSACCHARIDES [J].
BJORNDAL, H ;
HELLERQV.CG ;
LINDBERG, B ;
SVENSSON, S .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1970, 9 (08) :610-&
[3]  
BOUVENG HO, 1960, ADV CARBOHYD CHEM, V15, P53
[4]   DESICCANT EFFICIENCY IN SOLVENT DRYING .3. DIPOLAR APROTIC-SOLVENTS [J].
BURFIELD, DR ;
SMITHERS, RH .
JOURNAL OF ORGANIC CHEMISTRY, 1978, 43 (20) :3966-3968
[5]   A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].
CIUCANU, I ;
KEREK, F .
CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217
[6]   METHYLSULFINYLCARBANION [J].
COREY, EJ ;
CHAYKOVSKY, M .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1962, 84 (05) :866-&
[7]   AN IMPROVED METHOD FOR THE PREPARATION OF STANDARDS FOR GLYCOSYL-LINKAGE ANALYSIS OF COMPLEX CARBOHYDRATES [J].
DOARES, SH ;
ALBERSHEIM, P ;
DARVILL, AG .
CARBOHYDRATE RESEARCH, 1991, 210 :311-317
[8]   HEMICELLULOSIC POLYMERS FROM THE CELL-WALLS OF BEESWING WHEAT BRAN .1. POLYMERS SOLUBILIZED BY ALKALI AT 2-DEGREES [J].
DUPONT, MS ;
SELVENDRAN, RR .
CARBOHYDRATE RESEARCH, 1987, 163 (01) :99-113
[9]   SIMPLIFIED METHOD FOR THE MEASUREMENT OF TOTAL NON-STARCH POLYSACCHARIDES BY GAS - LIQUID-CHROMATOGRAPHY OF CONSTITUENT SUGARS AS ALDITOL ACETATES [J].
ENGLYST, HN ;
CUMMINGS, JH .
ANALYST, 1984, 109 (07) :937-942
[10]   SOLVATION AND ION-PAIRING OF ALKALI-METAL ALKOXIDES IN DIMETHYL-SULFOXIDE - CONDUCTOMETRIC STUDIES [J].
EXNER, JH ;
STEINER, EC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1974, 96 (06) :1782-1787