QUANTITATION OF PROPOFOL IN WHOLE-BLOOD BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY

被引：43

作者：

GUITTON, J

DESAGE, M

LEPAPE, A

DEGOUTE, CS

MANCHON, M

BRAZIER, JL

机构：

[1] CTR HOSP LYON SUD,BIOCHIM & TOXICOL LAB,F-69495 PIERRE BENITE,FRANCE

[2] CTR HOSP LYON SUD,SERV REANIMAT NORD,PIERRE BENITE,FRANCE

[3] CTR HOSP LYON SUD,SERV ANESTHESIE,PIERRE BENITE,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 669卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00105-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gas chromatographic-mass spectrometric assay, using selected-ion monitoring (GC-MS-SIM) with thymol as internal standard, was developed for quantitating propofol, an intravenous anaesthetic. The method described is rapid and sensitive for the determination of propofol in whole blood. The sensitivity of the present method is 10 ng/ml. The recovery of propofol added to human whole blood in the concentration range 10-10 000 ng/ml ranged between 95 and 100%. A single extraction procedure was used with chloroform-ethyl acetate. The assay allowed the detection of two metabolites formed during propofol metabolism: 2,6-diisopropyl-1,4-quinone and 2,6-diisopropyl-1,4-quinol.

引用

页码：358 / 365

页数：8

共 9 条

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AUDIBERT G, 1993, DRUG METAB DISPOS, V21, P7

[2]

BAKER MT, 1993, ANESTH ANALG, V76, P817

[3]

GEPTS E, 1987, ANESTH ANALG, V66, P1256

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