IDENTIFICATION AND CHARACTERIZATION OF THE SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE-D' CORE PROTEIN

被引:16
作者
ANDERSEN, J
FEENEY, RJ
ZIEVE, GW
机构
[1] SUNY STONY BROOK,DEPT PATHOL,STONY BROOK,NY 11794
[2] SUNY STONY BROOK,PROGRAM CELLULAR & DEV BIOL,STONY BROOK,NY 11794
关键词
D O I
10.1128/MCB.10.9.4480
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The addition of urea to sodium dodecyl sulfate (SDS)-polyacrylamide gels has allowed the identification and characterization of the small nuclear ribonucleoprotein particle (snRNP) D′ protein and has also improved resolution of the E, F, and G snRNP core proteins. In standard SDS-polyacrylamide gels, the D′ and D snRNP core proteins comigrate at approximately 16 kilodaltons. The addition of urea to the separating gel caused the D′ protein to shift to a slower electrophoretic mobility that is distinct from that of the D protein. The shift to a slower electrophoretic mobility in the presence of urea suggests that the D′ protein has extensive secondary structure that is not totally disrupted by SDS alone. Both N-terminal sequencing and partial peptide maps indicate that the D and D′ proteins are distinct gene products, and the sequence data have identified the faster moving of the two proteins as the previously cloned D protein (L. A. Rokeach, J. A. Haselby, and S. O. Hoch, Proc. Natl. Acad. Sci. USA 85:4832-4836, 1988). In the cytoplasm, the D protein is found primarily in the small-nuclear-RNA-free 6S protein complexes, while the D′ protein is found primarily in the 20S protein complexes. Like the D protein, the D′ protein is an autoantigen in patients with systemic lupus erythematosus and is recognized by some of the Sm class of autoimmune antisera.
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页码:4480 / 4485
页数:6
相关论文
共 24 条
[1]  
BILLINGS PB, 1982, J IMMUNOL, V128, P1176
[2]  
BILLINGS PB, 1985, J IMMUNOL, V135, P428
[3]  
BIRNSTIEL ML, 1988, STRUCTURE FUNCTION M
[4]   PURIFICATION OF THE INDIVIDUAL SNRNPS U1, U2, U5 AND U4/U6 FROM HELA-CELLS AND CHARACTERIZATION OF THEIR PROTEIN CONSTITUENTS [J].
BRINGMANN, P ;
LUHRMANN, R .
EMBO JOURNAL, 1986, 5 (13) :3509-3516
[5]  
CLEVELAND DW, 1977, J BIOL CHEM, V252, P1102
[6]   THE U2 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE AS AN AUTO-ANTIGEN - ANALYSIS WITH SERA FROM PATIENTS WITH OVERLAP SYNDROMES [J].
CRAFT, J ;
MIMORI, T ;
OLSEN, TL ;
HARDIN, JA .
JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (06) :1716-1724
[7]   NUCLEAR-EXCHANGE OF THE U1 AND U2 SNRNP-SPECIFIC PROTEINS [J].
FEENEY, RJ ;
ZIEVE, GW .
JOURNAL OF CELL BIOLOGY, 1990, 110 (04) :871-881
[8]  
FEENEY RJ, 1989, J BIOL CHEM, V264, P5776
[9]   SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE ASSEMBLY INVIVO - DEMONSTRATION OF A 6S RNA-FREE CORE PRECURSOR AND POSTTRANSLATIONAL MODIFICATION [J].
FISHER, DE ;
CONNER, GE ;
REEVES, WH ;
WISNIEWOLSKI, R ;
BLOBEL, G .
CELL, 1985, 42 (03) :751-758
[10]   SMALL NUCLEAR RNA-ASSOCIATED PROTEINS ARE IMMUNOLOGICALLY RELATED AS REVEALED BY MAPPING OF AUTOIMMUNE REACTIVE B-CELL EPITOPES [J].
HABETS, WJ ;
SILLEKENS, PTG ;
HOET, MH ;
MCALLISTER, G ;
LERNER, MR ;
VANVENROOIJ, WJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (12) :4674-4678