USE OF DETERGENTS IN THE PREPARATION OF SALMONELLA SAMPLES FOR ENZYME-IMMUNOASSAY ON POLYMYXIN-COATED POLYESTER CLOTH

被引：11

作者：

BLAIS, BW

YAMAZAKI, H

机构：

[1] CARLETON UNIV,DEPT BIOL,OTTAWA K1S 5B6,ONTARIO,CANADA

[2] CARLETON UNIV,INST BIOCHEM,OTTAWA K1S 5B6,ONTARIO,CANADA

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1990年 / 11卷 / 3-4期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CLOTH ENZYME IMMUNOASSAY; POLYMYXIN; DETERGENT; DEOXYCHOLATE; SALMONELLA; LIPOPOLYSACCHARIDE;

D O I：

10.1016/0168-1605(90)90026-2

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Heating Salmonella cells in the presence of detergents such as deoxycholate or Triton x-100 greatly increased the sensitivity of an enzyme immunoassay for lipopolysaccharide antigens using polymyxincoated polyester cloth as the solid phase for antigen capture. The presence of a 100-fold excess of E. coli lipopolysaccharide in the test sample did not affect the detection of the Salmonella lipopolysaccharide. The sensitivity of the assay using deoxycholate-heat treatment of the antigens followed by detection on polymyxin-coated cloth was equivalent to that of an assay using antibody-coated polyester cloth for antigen capture.

引用

页码：329 / 336

页数：8

共 3 条

[1]

BLAIS B W, 1989, Biotechnology Techniques, V3, P23, DOI 10.1007/BF01876216

[2] A predictive model for the non-thermal inactivation of Salmonella enteritidis in a food model system supplemented with a natural antimicrobial [J].

Koutsoumanis, K ;

Lambropoulou, K ;

Nychas, GJE .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 49 (1-2) :63-74

[3] USE OF POLYMYXIN-COATED POLYESTER CLOTH IN THE ENZYME-IMMUNOASSAY OF SALMONELLA LIPOPOLYSACCHARIDE ANTIGENS [J].

BLAIS, BW ;

YAMAZAKI, H .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1990, 11 (3-4) :195-204

← 1 →