PROTEIN ASSAY IN REVERSED MICELLE SOLUTIONS

被引：9

作者：

PIRES, MJ ^{[1
]}

PRAZERES, DMF ^{[1
]}

CABRAL, JMS ^{[1
]}

机构：

[1] Univ Tecn Lisboa, ENGN BIOQUIM LAB, INST SUPER TECN, P-1000 LISBON, PORTUGAL

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 04期

关键词：

D O I：

10.1007/BF00150901

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An adaptation of the Lowry (1951) method has been developed for quantifying proteins in organic reversed micellar solutions. Ethanol is added to improve the re-extraction of the proteins from the micelles and a centrifugation step is included to separate the organic phase.

引用

页码：293 / 294

页数：2

共 4 条

[1] SELECTIVE SEPARATION AND PURIFICATION OF 2 LIPASES FROM CHROMOBACTERIUM-VISCOSUM USING AOT REVERSED MICELLES [J].

AIRESBARROS, MR ;

CABRAL, JMS .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (11) :1302-1307

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[4] REVERSE MICELLES AS HOSTS FOR PROTEINS AND SMALL MOLECULES [J].

LUISI, PL ;

GIOMINI, M ;

PILENI, MP ;

ROBINSON, BH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 947 (01) :209-246

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