TRACE AMOUNTS OF TRITON X-100 MODIFY THE INHIBITOR SENSITIVITY OF THE MITOCHONDRIAL PORIN

被引：9

作者：

BATHORI, G ^{[1
]}

FONYO, A ^{[1
]}

LIGETI, E ^{[1
]}

机构：

[1] SEMMELWEIS UNIV MED,DEPT PHYSIOL,H-1444 BUDAPEST 8,HUNGARY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1995年 / 1234卷 / 02期

关键词：

PORIN; VDAC; HEXOKINASE; TRITON; POLYANION; CONTACT POINT;

D O I：

10.1016/0005-2736(94)00295-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transport properties of mitochondrial porin were investigated on the basis of changes in the activity of hexokinase utilizing external ATP. Production of glucose 6-phosphate is inhibited by polyanion both in intact brain mitochondria and in contact point vesicles. Hexokinase activity is restored by solubilization of the enzyme by high ionic strength or 0.5-1% Triton X-100. In very low concentrations (0.001-0.005%) Triton does not mobilize hexokinase from its binding sites but it is able to release polyanion-inhibition completely. This finding provides an explanation for the discrepancy observed in the transport properties of porin when studied 'in situ' or in artificial lipid membranes.

引用

页码：249 / 254

页数：6

共 26 条

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